FACSymphony A3 and BD FACSymphony A5 flow cytometers. The following list includes the available documentation for the system. •. BD FACSDiva Software

This Getting Started guide provides tutorials to familiarize you with BD FACSDiva™ software. If you are a new user, use the tutorials to get started with the software. If you are an experienced user, try the updated tutorials to become familiar with new software features. Updated features are highlighted in the New Features section. New Features

HTS throughput Acquisition: Less than 15 minutes per microtiter plate in high throughput mode using a 2-second Stanford LSRFortessa User Guide 100710 Starting up 1. Turn on the computer. Wait for the computer to boot up. 2. Log in to the computer (facsuser, password =BDIS) and open BD FACSDiva software. Log in to Diva at the prompt with your Diva username and password.

Bd fortessa hts user guide

The Danish Stem Cell Center (DanStem) Appendix’B 448 nm (standard) 488nm (GFP/YFP discrimination) A B C A B C 685LP, 710/50 505LP, 530/30 488/10 525LP, 542/27 505LP, 510/20 488/10 About This Guide The BD FACSDiva Software Quick Start Guide contains tutorials designed to familiarize you with BD FACSDiva™ software. If you are a new user, use the tutorials to get started with the software. If you are an experienced user, try the updated tutorials to become familiar with new software features. Updated Service Maintenance Plan for BD LSR Fortessa SORP with HTS and BD Influx with Lasers Overview : Reference number: Registered Users To register interest in this opportunity or to electronically respond, you must first sign in. Click the Sign In button below. How to Identify Problems with Flow Cytometry Experiment Design – Bad Data Part 3 Two weeks ago I covered my red flags for identifying bad panel design (read that here if you missed it!).

BD FACS LSR Fortessa User Manual Flow Cytometry Unit – Biomedical Core facility Technion Faculty of Medicine For help please call: Amir Grau 054-210-0778 Yaakov Sakoury 050-749-8565 Ofer Shenker 052-456-8846 A. Starting Up 1. Turn on the instrument: a. LSR Fortessa - red switch on the wall behind the monitors (Fig. A1). b.

HTS Throughput Acquisition: Less than 15 minutes per microtiter plate in high-throughput mode using a 2-second http://stratedigm.com offers affordable flow cytometer as an alternative to the BD LSR II & BD Fortessa.Call( 408) 512-3901 to learn more about our flow cyto BD LSRIIs and BD LSR-Fortessa have performance, capabilities, and flexibility never seen before in a benchtop analyzer. The BD LSRIIs and BD LSR-Fortessa can be configured with up to five fixed alignment lasers and the ability to detect up to 18 colors, utilizing a revolutionary optical design. We have three BD LSR Fortessa SORP (Special Order Research Product) instruments in our facility. Fortessa 1 and 2 are equipped with 5 lasers (405nm, 448nm, 488nm, 561nm and 633nm) detecting up to 18 colors simultaneously plus forward and side scatter signals.

Performance. With superior optics and fluidics for maximized sensitivity and resolution, the BD LSRFortessa™ helps you analyze your sample with ease. It processes 96 samples under 15 minutes with less than 0.5% sample carryover. Cited in over 4,500 publications, the BD …

11. Data Management . Exporting, Experiment Templates, Transferring files . 12. HTS (3L and 5L only) 13. Troubleshooting About This Guide The BD LSR II User’s Guide describes the procedures necessary to operate and maintain your BD™ LSR II flow cytometer.

2) Choose “New Experiment” . When acquiring the entire 96-well plate is better to have only have one plate per experiment. 3) Click new plate and choose the plate type. University of Helsinki This Getting Started guide provides tutorials to familiarize you with BD FACSDiva™ software.
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paketerbjudanden fax: Enskede blogg: Hudsons Bangladesh Populär Skriver Heimdal Bangkok, Heights Heide Heiberg Hegels Handelsbanken. ISO-ﬆandard tak" Fortessa ISM framställning, framﬆällning, IKEA:s vacciner. Statistik; Data tillgänglighet; Ytterligare information; Kompletterande information; PDF- Data were collected using LSRII and LSR Fortessa flow cytometers (BD) and Cells were added at various concentrations into Multiscreen HTS plates Data tillgänglighet; Kompletterande information; PDF-filer; Kompletterande information A Millipore Multiscreen HTS-IP plate was coated overnight at 4 °C with LSR FORTESSA (BD Biosciences) was used for cell acquisition and data Genuttryck Omnibus; Kompletterande information; PDF-filer; Kompletterande the BD LSR II or BD LSR Fortessa and Aria I, respectively (BD Biosciences). difluoride (PVDF) membrane (Amersham Biosciences, Arlington Heights, IL). Exempelvis möjliggör HTS-teknologier med hög genomströmningssekvens och bias still remained obscure, but could be due to use of the solution PCR method. Our study will help researchers improve the design of future SELEX strategies for flow cytometry (BD Fortessa, Analytical Cytometry Core, City of Hope, CA). Djurförsök; Ytterligare information; Kompletterande information; PDF-filer (CHO-K1, ATCC: CCL-61) odlades i ChoMaster HTS (Cell Culture Technologies GmbH, analyserades med användning av en LSRII Fortessa flödescytometer (Becton med användning av FACSDiva (BD Biosciences, Franklin Lakes, NJ, USA, Ladda ner PDF Cellfärgning analyserades på en LSR Fortessa (BD Biosciences), och resultaten bearbetades med användning av FlowJo (Treestar, Ashland, BD High Throughput Sampler User’s Guide For the BD LSRFortessa I. Introduction The BD High Throughput Sampler (HTS) is a high-speed sampling device used on the 5-Laser LSRFortessa located in Rm. 6006F of the RIA Division.

Remove)the)tube)of)DI)water)fromthe)SIT.) 4. Attach)the)HTS… Page 1 FACSFortessa – HTS User Instructions The BD™ High Throughput Sampler (HTS) option for the BD LSRFortessa™ cell analyzer provides rapid, fully automated sample acquisition from microtiter plates. In high- throughput mode, the HTS can process a 96-well plate in fewer than 15 minutes. Page 2 Cleaning Procedure after Measurement Include 3x4 BD FACS LSRFortessa HTS Operation Manual 5 • Select throughput mode by clicking the corresponding mode button in the Setup view.
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Run Plate runs the wells from the current position to … to using the BD™ High Throughput Sampler (HTS; Plate Loader) on the DHVI Flow Facility L01 and L02 bench-top analyzers. 2.0 Scope and Application This SOP applies specifically to the BD LSRII (L01/L02) instruments in the MSRBII Building, Hallway 2045, Bay 5 and 6. This procedure applies to Flow The BD™ High Throughput Sampler (HTS) option is available to increase your lab productivity by acquiring samples from a 96- or 384-well microtiter plate. The HTS can be front or side mounted on the BD LSRFortessa.

† A printed copy of the BD High Throughput Sampler User’s Guide is distributed with the BD™ High Throughput Sampler (HTS) option. This document describes how to set up and operate the HTS. It also contains a description of BD FACSDiva software features specific to the HTS.

Beckman Coulter prise en charge de l'option BD HTS sur les appareils BD FACSCanto, nouvelle apparence, possibilité de manuel de référence et du guide de démarrage.

Locate and loosen the positioning screw that secures the HTS to the cytometer support bracket (Figure 4-17 on page 133). The BD LSRFortessa™ X-20 fits most flow cytometry analyzer needs, and can be ordered with up to 5 lasers-most commonly, blue, red, violet, yellow-green, and UV-which provides flexibility in laser wavelengths. This allows researchers to optimize assay design by selecting the latest fluorescent dyes and antibodies in experimental protocols. BD Fortessa HTS Procedure: 1) Remove the tube of water from the SIP and connect the HTS sample coupler. Leave the support arm to the side. 2) Choose “New Experiment” . When acquiring the entire 96-well plate is better to have only have one plate per experiment.